transient receptor potential canonical 1 trpc1  (Santa Cruz Biotechnology)

Journal: Biological Research

Article Title: Shear stress-induced Ca 2+ influx triggers endoplasmic reticulum stress and cardiomyocyte apoptosis: implications for mitral regulation

doi: 10.1186/s40659-026-00671-4

Figure Lengend Snippet: The effects of TRPC1 knockdown on real-time Ca 2+ measurements. A To optimize the TRPC1 knockdown efficiency, HL-1 cells were transfected with siCtrl, siTRPC1#1, or siTRPC1#2 for 24 and 48 h. Parental cells were not transfected with siRNA. B Images were taken at 1 frame/3 s to record Ca 2+ activity in 2 mM Ca 2+ buffer. HL-1 cells were pretreated with fura-2 (2 μM) for 30 min. The panels are the Ca 2+ curve of the conditions of siCtrl, siCtrl with shear stress, siTRPC1 and siTRPC1 with shear stress. C The columns represent the amplitude of the Ca 2+ oscillations and D the changes in the Ca 2+ level in each group. All columns are presented as the mean ± SEM of at least three independent experiments with at least 20 cells of each group. The results were analyzed via one-way ANOVA. *** p < 0.001

Article Snippet: The samples were analyzed using antibodies against Grp78 (BD, 610978), ATF6 (Santa Cruz, sc166659), p-PERK (Cell Signaling, 3179), PERK (Cell Signaling, 3192), p-IRE1α (Abcam, ab48187), IRE1α (Abcam, ab37073), p-eIF2α (Cell Signaling, 9721), eIF2α (Cell Signaling, 9722), ATF4 (Santa Cruz, sc390063), CHOP (Cell Signaling, 2895), Bax (Genetex, GTX109683), Bcl-2 (BD, 610538), Bak (Cell Signaling, 12105), caspase 9 (Santa Cruz, sc56076), caspase 12 (Cell Signaling, 35965), caspase 3 (Abcam, ab32042), caspase 8 (Proteintech, 13423-1-AP), Bid (Novus, 10988-1-AP), and transient receptor potential canonical 1 (TRPC1) (Santa Cruz, sc133076).

Techniques: Knockdown, Transfection, Activity Assay, Shear

Journal: Bioengineering

Article Title: Synergistic Cellular Responses Conferred by Concurrent Optical and Magnetic Stimulation Are Attenuated by Simultaneous Exposure to Streptomycin: An Antibiotic Dilemma

doi: 10.3390/bioengineering11070637

Figure Lengend Snippet: List of antibodies, vendors, and dilution factors.

Article Snippet: Transient receptor potential canonical 1 (TRPC1) , Santa Cruz Biotechnology, Dallas, TX, USA , 1:500.

Techniques:

Journal: Bioengineering

Article Title: Synergistic Cellular Responses Conferred by Concurrent Optical and Magnetic Stimulation Are Attenuated by Simultaneous Exposure to Streptomycin: An Antibiotic Dilemma

doi: 10.3390/bioengineering11070637

Figure Lengend Snippet: Myogenic proliferation associated protein expression in response to exposure to light, magnetic fields, and their combination. ( A ) Protein expression of ( i ) cyclin D1, ( ii ) TRPC1, ( iii ) phosphorylated ERK, ( iv ) and cyclin B1 in response to the indicated exposure intervention (n = 3) in the absence of streptomycin. ( B ) Protein expressions of ( i ) cyclin D1, ( ii ) TRPC1, ( iii ) phosphorylated ERK, ( iv ) and cyclin B1 either in the presence of streptomycin (100 µg/mL) during (blue shaded box) or after (red shaded box) exposure as indicated (n = 3). The shaded areas indicate the absence of streptomycin (gray) or its application before and during (blue) or after exposure (red) to the indicated conditions. All data shown in panel ( B ) were collected from cells of the same plating and represent independent cell samples as those used in panel ( A ). Statistical analyses were performed minimally in three independent biological replicates. Data were analyzed using one-way ANOVA followed by multiple comparison tests. Significance levels are indicated as follows: * p < 0.05 and ** p < 0.01. The error bars represent the standard error of the mean (SEM).

Article Snippet: Transient receptor potential canonical 1 (TRPC1) , Santa Cruz Biotechnology, Dallas, TX, USA , 1:500.

Techniques: Expressing, Comparison

Journal: Biochimica et biophysica acta

Article Title: Involvement of store-operated Ca(2+) entry in activation of AMP-activated protein kinase and stimulation of glucose uptake by M3 muscarinic acetylcholine receptors in human neuroblastoma cells.

doi: 10.1016/j.bbamcr.2014.09.012

Figure Lengend Snippet: Fig. 7. mAChR stimulation induces STIM1–Orai1 and TRPC1–Orai1 interactions. (A) Cells were pre-treated with either vehicle or 100 nM atropine (atrop) for 5 min and then exposed to vehicle or 10 μM CCh for 5 min. Cell lysates were incubated with anti-Orai1 antibody and STIM1 and Orai1 immunoreactivities were analyzed in immunoprecipitates and cell lysates. STIM1 immunoreactivity in immunoprecipitates (IP) was normalized to that present in cell lysates. Values are expressed as percent of control and are the mean ± SEM of four experiments. *P b 0.05 vs control (vehicle), #P b 0.05 by ANOVA followed by Newman–Keuls post hoc test. (B) Cells were treated with either vehicle or CCh as described in (A) and cell lysates were subjected to immunoprecipitation with pre-immune IgG. Immunoprecipitates and cell lysates were then analyzed for STIM1 and Orai1 immunoreactivities. The image shows immunoblots of sample pair (vehicle and CCh) from two independent experiments. (C) Cells were treated with either vehicle or CCh as in (A) and cell lysates were immunoprecipitated with anti-Orai1 antibody. Immunoprecipitates and cell lysates were analyzed for TRPC1 and Orai1 immunoreactivities. The amount of TRPC1 in immunoprecipitates was normalized to that present in cell lysates. Values are expressed as percent of control and are the mean ± SEM of three experiments. *P b 0.05 vs control (vehicle). (D) Cells were treated with either vehicle or CCh as in (A) and cell lysates were immunoprecipitated with anti-TRPC1 antibody. Immunoprecipitates and cell lysates were analyzed for STIM1 and TRPC1 immunoreactivities. The image shows immunoblots of sample pair (vehicle and CCh) from two independent experiments and is representative of a total of four experiments.

Article Snippet: The supernatants (~700 μg of protein) were incubated overnight at 4 °C with either antiOrai1 (3 μg), anti-transient receptor potential canonical 1 (TRPC1) (2 μg) antibodies or pre-immune IgG (3 μg) (Santa Cruz Biotechnology).

Techniques: Incubation, Control, Immunoprecipitation, Western Blot